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KMID : 0525720170220040241
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Journal of Chitin and Chitosan
2017 Volume.22 No. 4 p.241 ~ p.247
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Sulfated Polysaccharide Protects against Ionizing Radiation-induced Skin Damages
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Kim A-Reum
Cho Jin-Hee
Herath Kalahe Hewage Iresha Nadeeka Madushani
Jee Young-Heun
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Abstract
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Sulfated polysaccharide (SP), generally known as fucoidan and commonly found in the cell walls of brown algae, has been reported to present biological effects including anti-inflammatory, anticancer, and antioxidant activities. Exposure to ionizing gamma-ray induces cellular production of reactive oxygen species and hair follicular cells, being the most sensitive to gammaray immediately go through apoptosis and damage their rapid differentiation essential for regeneration of hair. In this study we investigated the protective potential of SP’s against gamma-ray induced hair follicle cells injury. For that, 8.5Gy gamma-ray was irradiated to the whole body of C57BL/6 mouse at day 6 after depilation treated with and without SP. Treatment with SP markedly inhibited formation of thiobarbituric acid-reactive substances(TBARS) compared to control group (without SP) after 48 h of irradiation. Increased cleaved caspase-3 and p53 expression by irradiation was decreased by treatment with SP after 8 h of irradiation. Furthermore, terminal deoxyribonucleotidyl transferase-mediated d-uridine 5' triphosphate nick end labelling (TUNEL) staining showed that the proportion of apoptotic cells in gamma-ray irradiated skins was significantly lower in mouse treated with SP than control group (without SP). Also, SP treatment increased the percentage of Ki-67 positive cells compared with those of irradiation only group. These results suggest that SP has potential as a radioprotector for hair follicular cells.
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KEYWORD
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Seaweed, Sulfated polysaccharide, Gamma-rays, Oxidative stress, Radioprotection, Hair follicular cells
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